human brain cancer cell lines u87  (Korean Cell Line Bank)

Journal: Cells

Article Title: Non-Thermal Biocompatible Plasma Jet Induction of Apoptosis in Brain Cancer Cells

doi: 10.3390/cells10020236

Figure Lengend Snippet: Effects of NBP-J on cell viability of ( a ) U87 MG, ( b ) astrocytes, ( c ) U251, and ( d ) HCT116 cell lines for different treatment durations from 10 to 180 s. An Alamar blue assay was performed after 24 h of treatment. Data are presented as means ± SD of three independent sets of experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: U87 MG and U251 (human brain glioblastoma cancer cell line) and HCT-116 (human colon cancer cell line) were obtained from the Korean Cell Line Bank (KCLB, Seoul, Korea), and normal human astrocytes were obtained from Lonza (CC-2565, Basel, Switzerland).

Techniques: Alamar Blue Assay

Journal: Cancers

Article Title: Non-Thermal Atmospheric Pressure Bio-Compatible Plasma Stimulates Apoptosis via p38/MAPK Mechanism in U87 Malignant Glioblastoma

doi: 10.3390/cancers12010245

Figure Lengend Snippet: ( A ) Morphological changes in cell lines of astrocytes and U87 MG cells treated with plasma for 30, 60, and 180 s and viewed under a light microscope. Arrows indicate cell shrinkage and nuclear condensation due to apoptosis and the presence of apoptotic bodies. ( B ) SEM morphology of astrocytes and U87 MG cells. Effects of a soft plasma jet on cell viability of astrocytes ( C ) and U87 MG cells ( D ). ( E ) Confocal microcopy images of intracellular reactive oxygen species (ROS) and intracellular reactive nitrogen species (RNS) of U87 MG cells. ( F ) Relative amounts of ROS and RNS levels following soft plasma jet treatment of U87 MG cells. Scale bar = 100 μm. All values are presented as means ± SD of three independent experiments. ** p < 0.01, and *** p < 0.001.

Article Snippet: U87 MG (human brain cancer cell line) and normal human astrocytes (NHA) were purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea) and Lonza (CC-2565, Basel, Switzerland), respectively.

Techniques: Clinical Proteomics, Light Microscopy

Journal: Cancers

Article Title: Non-Thermal Atmospheric Pressure Bio-Compatible Plasma Stimulates Apoptosis via p38/MAPK Mechanism in U87 Malignant Glioblastoma

doi: 10.3390/cancers12010245

Figure Lengend Snippet: Apoptosis in astrocytes and U87 MG cells after plasma treatment: ( A ) representative flow cytometry (fluorescence-activated cell sorting (FACS)) dot plots of astrocytes and U87 MG cells prepared using the Annexin V-FITC Apoptosis Detection Kit; ( B ) summaries of frequencies of early and late apoptosis events for astrocytes and U87 MG cells; ( C ) effects of a soft plasma jet on the cell cycle distribution of U87 MG human brain cancer cells; ( D ) the percentage of cells in different cell cycle phases. The cells were treated for 30, 60, and 180 s and analyzed by flow cytometry. All values are presented as means ± SD of three independent experiments. ** p < 0.01, and *** p < 0.001.

Article Snippet: U87 MG (human brain cancer cell line) and normal human astrocytes (NHA) were purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea) and Lonza (CC-2565, Basel, Switzerland), respectively.

Techniques: Clinical Proteomics, Flow Cytometry, Fluorescence, FACS

Journal: Cancers

Article Title: Non-Thermal Atmospheric Pressure Bio-Compatible Plasma Stimulates Apoptosis via p38/MAPK Mechanism in U87 Malignant Glioblastoma

doi: 10.3390/cancers12010245

Figure Lengend Snippet: ( A ) Western blot analysis of protein expression in U87 MG cells; ( B ) relative band intensity as a function of treatment time.

Article Snippet: U87 MG (human brain cancer cell line) and normal human astrocytes (NHA) were purchased from the Korean Cell Line Bank (KCLB, Seoul, Korea) and Lonza (CC-2565, Basel, Switzerland), respectively.

Techniques: Western Blot, Expressing